21.(7分,除标注外,每空1分)(1)启动子运载体(2)促性腺(3)pH内细胞团(4)检测到相应蛋白质和出现高血压(2分)【解析】(1)由mRNA反转录产生的cDNA片段中不含启动子和终止子,因此构建基因表达载体时需在其上游添加启动子,而质粒则是作为运载体2)为了获得更多的卵子,需用促性腺激素对雌鼠进行处理,使其超数排卵(3)体外培养受精卵时,培养液中的CO2可以维持培养液的pH;可从培养至囊胚期的早期胚胎的内细胞团中分离胚胎干细胞(4)若在子代大鼠的体内检测出与高血压相关的蛋白质,则从分子水平上说明高血压相关基因已成功表达;若子代大鼠的血压升高,则从个体水平上说明高血压相关基因已成功表达。
第二节One possible versionniceDear overseas students from America,Here comes the news that Chinese Bridge series activities will be held in our school. So all of participants arewarmly welcome in the pep rally to get fully prepared for thefolloThemed at promoting Chinese culture around theworld, Chinese Bridge acts as a platform to help narrow thegap of mutual understanding about China. Whoever isactively involved in it will develop a positive awareness ofChinese and its culture behind. Based on it, the pep rallis scheduled in our school auditorium from 14: 00 p.m15: 00 P. m. next Friday afternoonPlease make sure you are available at that time andThe student’ s Union
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